Live cell imaging of fluorescent proteins has become a core technique in molecular cell biology. Our group pioneered this approach in plant pathogenic fungi and provided insight into fundamental principles of fungal cell organisation and pathogenicity. The recent development of a live cell imaging toolset for the wheat pathogen Zymoseptoria tritici has enabled us to take a more applied approach, using cell biological methods to understand the way by which anti-fungal chemistries affect the pathogen (fungicide mode of action). In this talk, I shall provide our unpublished results on such investigations, using well-established fungicides. I shall demonstrate the power of this novel experimental strategy and provide insight into how live cell imaging can help to develop new fungicides, needed to secure our future food supplies.