Plant-parasitic nematodes are a major constraint to sugarcane production in Australia. Previous research has found that the wild canes Erianthus arundinaceus and some of its crosses with commercial sugarcane have resistance to both root-knot nematode (Meloidogyne javanica) and root lesion nematode (Pratylenchus zeae). No information was available on Saccharum spontaneum clones, a close relative of modern sugarcane, on their resistance to sugarcane nematodes. Thirteen wild clones of S. spontaneum screened two times against root knot nematodes and one time against root lesion nematodes in 2012 and 2013 in a glasshouse at SRA Woodford Pathology farm. Further, in 2016 to 2017, 20 basic S. spontaneum clones from SRA Meringa germplasm collection were screened against both types of nematodes. Nematode reproduction (both M. javanica and P. zeae) were significantly lower on basic S. spontaneum clones compared to backcross progenies and commercial standards in all trials. Some progenies from S. spontaneum and commercial varieties also had low nematode reproduction. This study provided a valuable information on future development of nematode resistance variety for the Australian sugar industry. Details of the results will be presented.